How many AI startups in the U.S. have raised over $100 million in 2025?

As of June 2025, 24 AI startups in the U.S. have raised funding rounds worth $100 million or more.

Which AI startup raised the most funding in 2025?

OpenAI raised the most in 2025 so far, closing a $40 billion round led by SoftBank, valuing the company at $300 billion.

Are AI startups still getting funded in 2025?

Yes, AI funding remains strong in 2025, with more billion-dollar rounds in Q1 compared to the same period in 2024.

Top 24 U.S. AI Startups That Raised $100M+ in 2025 (So Far)

The AI investment boom shows no signs of slowing down in 2025. Just halfway into the year, 24 U.S.-based AI startups have already raised funding rounds worth $100 million or more — with several hitting billion-dollar valuations.

While 2024 set a high benchmark with 49 such mega-rounds, 2025 has already seen more $1B+ rounds in Q1 alone. Let’s take a look at the biggest AI funding stories of the year (so far), categorized by month.

???? June 2025

Glean raised $150M Series F — now valued at $7.25B
Anysphere (Cursor) raised a $900M Series C, nearly $10B valuation

???? May 2025

Snorkel AI secured a $100M Series D ($1.3B valuation)
LMArena, the AI benchmark platform, raised $100M seed (valued at $600M)
TensorWave, AI infra startup, raised $100M Series A

???? April 2025

SandboxAQ landed $450M Series E ($5.7B valuation)
Runway raised $308M Series D, valued at $3B

???? March 2025

OpenAI broke records with a $40B round ($300B valuation)
Nexthop AI raised $110M Series A
Insilico Medicine raised $110M Series E
Celestial AI landed $250M Series C ($2.5B valuation)
Lila Sciences raised $200M seed round
Reflection.Ai secured $130M Series A
Turing raised $111M Series E
Shield AI closed $240M Series F ($5.3B valuation)
Anthropic raised $3.5B Series E, now worth $61.5B

???? February 2025

Together AI raised $305M Series B
Lambda secured $480M Series D
Abridge closed a $250M Series D ($2.75B valuation)
Eudia raised $105M Series A
EnCharge AI raised $100M Series B
Harvey raised $300M Series D ($3B valuation)

????️ January 2025

ElevenLabs raised $180M Series C ($3B+ valuation)
Hippocratic AI closed a $141M Series B ($1.6B valuation)

???? Why This Matters

This wave of funding underlines one key trend: AI is no longer emerging tech — it’s foundational. Startups across healthcare, infrastructure, law, voice, and enterprise tools are drawing serious venture capital interest.

If you’re building in AI or following the space closely, don’t miss our guide on how to launch your own AI SaaS startup in 2025.